| Gene | Glt6d1 |
| Chromosome | 2 |
| Deletion Start | 25,814,195 |
| Deletion End | 25,794,037 |
| Deletion Size | 20,159 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,812,881 | 25,812,905 | AGGATTTAGTCTAGCCCATGTTGTG |
| TUP | 25,812,855 | 25,812,879 | CCAGCCCCTGGAGTCTCCAGTCAGA |
| TUR | 25,812,825 | 25,812,846 | CAACACCCAACTCACCCTGTAG |
| TDF | 25,794,436 | 25,794,458 | ATGGTCTTCCAAAGTGAGTTTGG |
| TDP | 25,794,393 | 25,794,418 | CATCTGTGGCTCAGCTCCATCCTTGG |
| TDR | 25,794,370 | 25,794,391 | TTCGTCCTCTTTCGGAAATACC |
PCR Primers
| Primer | Sequence |
| SU | ACGGATTTGCTTCCCCAAG |
| SD | TGTGTCTCACTAACATCACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 334 bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | 329 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10185A-A3 | VGB6 | C57BL/6NTac | |
| 10185A-B1 | VGB6 | C57BL/6NTac | |
| 10185A-C5 | VGB6 | C57BL/6NTac | |
| 10185A-C11 | VGB6 | C57BL/6NTac | |
| 10185A-F4 | VGB6 | C57BL/6NTac | |
| 10185A-G5 | VGB6 | C57BL/6NTac | |
