| Gene | Ptrh1 |
| Chromosome | 2 |
| Deletion Start | 32,775,814 |
| Deletion End | 32,777,304 |
| Deletion Size | 1,491 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,776,483 | 32,776,501 | GCGCCTCATGAACGTCAAC |
| TUP | 32,776,508 | 32,776,534 | CTACAACTCACCAGCTCGGGCTACGCT |
| TUR | 32,776,542 | 32,776,562 | GGTCCCTGTGCCCTCTTATCC |
| TDF | 32,776,959 | 32,776,982 | AACTGCTCTTCATATCCCCTCAAG |
| TDP | 32,776,991 | 32,777,020 | TCAGACAGCTAATGCAGGAACGGACTCCAT |
| TDR | 32,777,024 | 32,777,046 | GCAGAAGGGTAGACTCACATTGG |
PCR Primers
| Primer | Sequence |
| SU | GAGCAAAGGCTGAGCCATAC |
| SD | AAAAGGTTCAGAAAGTTTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 357 bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | 326 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10186A-A4 | VGB6 | C57BL/6NTac | |
| 10186A-A8 | VGB6 | C57BL/6NTac | |
| 10186A-B2 | VGB6 | C57BL/6NTac | |
| 10186A-B4 | VGB6 | C57BL/6NTac | |
| 10186A-B12 | VGB6 | C57BL/6NTac | |
| 10186A-E5 | VGB6 | C57BL/6NTac | |
