| Gene | Lcn12 |
| Chromosome | 2 |
| Deletion Start | 25,493,853 |
| Deletion End | 25,490,905 |
| Deletion Size | 2,949 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,493,762 | 25,493,782 | TCAAGGCTTTCCCCAGATGAC |
| TUP | 25,493,732 | 25,493,760 | AAGCCCTGTACCTGGTCACTTTGGAAGCT |
| TUR | 25,493,706 | 25,493,725 | GCTGCCACCTGCTTCTTCTC |
| TDF | 25,491,091 | 25,491,111 | TGCCACTCTACAGGGCATGTC |
| TDP | 25,491,065 | 25,491,086 | AAACCCCTCCCTGCAGCCGCTT |
| TDR | 25,491,037 | 25,491,059 | TGGAAGTTAGGCAGGGAGATGAG |
PCR Primers
| Primer | Sequence |
| SU | GTTGGGTCCAGTAAGTTTTC |
| SD | GTCCATTATGTCCACCTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 341 bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | 321 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10188A-A11 | VGB6 | C57BL/6NTac | |
| 10188A-C7 | VGB6 | C57BL/6NTac | |
| 10188A-G8 | VGB6 | C57BL/6NTac | |
| 10188A-H12 | VGB6 | C57BL/6NTac | |
