| Gene | Fcnb |
| Chromosome | 2 |
| Deletion Start | 28,084,854 |
| Deletion End | 28,076,575 |
| Deletion Size | 8,299 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 28,084,434 | 28,084,456 | GGTACATATGGGCAAGGATTTGG |
| TUP | 28,084,390 | 28,084,417 | TGATCAGCTCTGCTCTGATATCCCCTGG |
| TUR | 28,084,358 | 28,084,379 | AGACAGTTGCCCTGTGCTTAGG |
| TDF | 28,076,916 | 28,076,938 | CGTACTCTCTCCCAAGGCTTATG |
| TDP | 28,076,890 | 28,076,914 | AGCTGGGTCCCTCACCTGGATCTCA |
| TDR | 28,076,866 | 28,076,888 | TCCTCGATTGGAGGATTTATCAG |
PCR Primers
| Primer | Sequence |
| SU | CTAGACCTGGCTCAGATGCC |
| SD | AACCCTTCCTGACTGTCAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 352 bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | 396 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10190A-A9 | VGB6 | C57BL/6NTac | |
| 10190A-E11 | VGB6 | C57BL/6NTac | |
| 10190A-F1 | VGB6 | C57BL/6NTac | |
| 10190A-F10 | VGB6 | C57BL/6NTac | |
| 10190A-G10 | VGB6 | C57BL/6NTac | |
