| Gene | Sardh |
| Chromosome | 2 |
| Deletion Start | 27,244,499 |
| Deletion End | 27,211,023 |
| Deletion Size | 33,499 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 27,244,434 | 27,244,454 | GGGAGAGCTGCTTGGAATCTG |
| TUP | 27,244,404 | 27,244,428 | ACAACCCCTAGCCACAGAGGCCAGG |
| TUR | 27,244,367 | 27,244,387 | GGGTCCGCTGATAAGGAACAC |
| TDF | 27,211,278 | 27,211,300 | TGACCATCCTGTAGCGAGAACTC |
| TDP | 27,211,252 | 27,211,276 | CCTATAGCCCGGCTTCCCTGACAGC |
| TDR | 27,211,216 | 27,211,238 | CAAGTGCCACCATATGAGAAAGC |
PCR Primers
| Primer | Sequence |
| SU | AGTGGGGGAGTAAGTAAACAC |
| SD | GAGAGCCCCCGGAGTCCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 283 bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | 423 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10194A-D1 | VGB6 | C57BL/6NTac | |
| 10194A-D7 | VGB6 | C57BL/6NTac | |
