| Gene | Slc2a6 |
| Chromosome | 2 |
| Deletion Start | 27,027,902 |
| Deletion End | 27,021,895 |
| Deletion Size | 6,008 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 27,027,232 | 27,027,253 | GCAATTTCAGCTTTGGGTATGC |
| TUP | 27,027,194 | 27,027,222 | CACATCTCCAGTCATCCCTGAACTGAAGC |
| TUR | 27,027,170 | 27,027,192 | GGTGTAGTGCTGGGTCAGAAGAG |
| TDF | 27,022,370 | 27,022,388 | TCCCAGGGAAATGCAGATG |
| TDP | 27,022,340 | 27,022,365 | AGCAATAAGCTGGAACCTGCCCTGCC |
| TDR | 27,022,317 | 27,022,335 | CTCTCAACCGTGGCCATTG |
PCR Primers
| Primer | Sequence |
| SU | TAAAGCCAGACCCAGCCATG |
| SD | GCCTGCTCCCCCTGCAGATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 430 bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 282 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10195A-D11 | VGB6 | C57BL/6NTac | |
| 10195A-E12 | VGB6 | C57BL/6NTac | |
