| Gene | Gbgt1 |
| Chromosome | 2 |
| Deletion Start | 28,497,746 |
| Deletion End | 28,505,395 |
| Deletion Size | 7,650 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 28,498,551 | 28,498,572 | GGCTCTGAGCAGTGCTCCTTAG |
| TUP | 28,498,575 | 28,498,600 | CTGCCGTAGACTCGAGCTGAGAACCG |
| TUR | 28,498,603 | 28,498,625 | CAGTATGTTGGAAGGCTGGAATC |
| TDF | 28,504,870 | 28,504,888 | TTCCCGGTGGGAGGAGATC |
| TDP | 28,504,891 | 28,504,913 | CATGCGCCGAATGGAGACCATCA |
| TDR | 28,504,919 | 28,504,939 | GTGGGCTCTCTTGGCAATGTG |
PCR Primers
| Primer | Sequence |
| SU | TGGCCAGAAGTAGCTTTAGG |
| SD | TGGATTCCCCAGCCTGCCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 422 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | 336 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10196G-B1 | VGB6 | C57BL/6NTac | |
| 10196G-B12 | VGB6 | C57BL/6NTac | |
| 10196G-H7 | VGB6 | C57BL/6NTac | |
| 10196H-A9 | VGB6 | C57BL/6NTac | |
| 10196H-D6 | VGB6 | C57BL/6NTac | |
| 10196H-D8 | VGB6 | C57BL/6NTac | |
