| Gene | Ppp1r26 |
| Chromosome | 2 |
| Deletion Start | 28,450,344 |
| Deletion End | 28,453,851 |
| Deletion Size | 3,508 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 28,450,627 | 28,450,647 | TGCAAAGAGCAGCCAGAGTTC |
| TUP | 28,450,653 | 28,450,676 | CTGCAGTTTGCCACGAGACCACGG |
| TUR | 28,450,690 | 28,450,708 | TGCGTCTCCCAGCTTCATC |
| TDF | 28,453,719 | 28,453,740 | GAGATATCGGCACAGGGTTGAC |
| TDP | 28,453,745 | 28,453,768 | CCAAGGTCTCCTGGTCCTGCGGTT |
| TDR | 28,453,772 | 28,453,792 | CACTGAATTCGCTGGCATCAC |
PCR Primers
| Primer | Sequence |
| SU | TTTTTAACTGAGTCTTCCACC |
| SD | TTTACAAAAAACCTACAAAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 353 bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | 447 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10197C-C12 | VGB6 | C57BL/6NTac | |
| 10197D-C11 | VGB6 | C57BL/6NTac | |
