| Gene | Trim44 |
| Chromosome | 2 |
| Deletion Start | 102,400,681 |
| Deletion End | 102,400,001 |
| Deletion Size | 681 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 102,400,144 | 102,400,165 | AGAGTGGCCAAGAGGAAGTGTC |
| TUP | 102,400,116 | 102,400,142 | TAGGTGCTCAAATCCAGCCCATGGTCT |
| TUR | 102,400,091 | 102,400,113 | GATGAGCTGCCTATCTTCCTGAC |
| TDF | 102,400,146 | 102,400,167 | AGAGAGTGGCCAAGAGGAAGTG |
| TDP | 102,400,117 | 102,400,143 | AGGTGCTCAAATCCAGCCCATGGTCTG |
| TDR | 102,400,082 | 102,400,105 | CAGGACACAGATGAGCTGCCTATC |
PCR Primers
| Primer | Sequence |
| SU | GCCCAGCGCTGACGGCTG |
| SD | GGCGTCACAGTTCTCAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 312 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | 361 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10200A-C5 | VGB6 | C57BL/6NTac | |
| 10200A-C7 | VGB6 | C57BL/6NTac | |
| 10200A-E5 | VGB6 | C57BL/6NTac | |
