| Gene | Cd302 |
| Chromosome | 2 |
| Deletion Start | 60,284,117 |
| Deletion End | 60,252,271 |
| Deletion Size | 31,847 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 60,283,967 | 60,283,985 | ACCATCCCGCAACCATCTC |
| TUP | 60,283,929 | 60,283,951 | TCAGAGAGCCAGACCCGGGACAA |
| TUR | 60,283,888 | 60,283,909 | GGATACAGACACGGTGGAAGTG |
| TDF | 60,253,154 | 60,253,175 | ATACTCCCACCACCACGTTCTC |
| TDP | 60,253,125 | 60,253,151 | CCTTCCCTCCAAAGGTCTATCTCGCCA |
| TDR | 60,253,100 | 60,253,123 | GCAAGACAAGAGTCCAGTGTTTGG |
PCR Primers
| Primer | Sequence |
| SU | TATCTTTAACGAAGAGAAG |
| SD | AGAAGGTTAATGTTTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 295 bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | 235 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10205B-A6 | VGB6 | C57BL/6NTac | |
| 10205B-D3 | VGB6 | C57BL/6NTac | |
