| Gene | Gcg |
| Chromosome | 2 |
| Deletion Start | 62,480,553 |
| Deletion End | 62,474,959 |
| Deletion Size | 5,595 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 62,480,386 | 62,480,405 | TTGGTGAGTAGGGTCCCAGG |
| TUP | 62,480,355 | 62,480,382 | CCACTGTGTGTTCAATACGTCCCTCCAA |
| TUR | 62,480,327 | 62,480,346 | CGTATCCCAGATCAGCCCAC |
| TDF | 62,475,792 | 62,475,812 | CTGATGGCTCCTTCTCTGACG |
| TDP | 62,475,760 | 62,475,788 | TGAGCACCATTCTGGATAATCTTGCCACC |
| TDR | 62,475,738 | 62,475,758 | TCAGCCAGTTGATGAAGTCCC |
PCR Primers
| Primer | Sequence |
| SU | AGAAGTGTCCTACATTAATG |
| SD | TGAGGAGAAAAAAATATGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 381 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10206A-A5 | VGB6 | C57BL/6NTac | |
| 10206A-G10 | VGB6 | C57BL/6NTac | |
| 10206B-B10 | VGB6 | C57BL/6NTac | |
| 10206B-F12 | VGB6 | C57BL/6NTac | |
