| Gene | Tmx2 |
| Chromosome | 2 |
| Deletion Start | 84,678,036 |
| Deletion End | 84,672,367 |
| Deletion Size | 5,670 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 84,677,898 | 84,677,917 | ACTGCCACCGATTTGCAATG |
| TUP | 84,677,865 | 84,677,886 | CAACGCGAAGATGGCAACCCGT |
| TUR | 84,677,843 | 84,677,862 | GCGCTCACCCAGTCAAAGTC |
| TDF | 84,672,573 | 84,672,596 | TGCTCCTGCTCTCTGAACTTTACC |
| TDP | 84,672,546 | 84,672,571 | TCTCACCCAGCCGTTGATGTGTTCAT |
| TDR | 84,672,515 | 84,672,538 | TTCTCGAATCACATTCTCCTATGC |
PCR Primers
| Primer | Sequence |
| SU | TGAGAGACTGGTTAGGAAGG |
| SD | TCTATCGATAAGATCACTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 368 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | 320 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10209A-A10 | VGB6 | C57BL/6NTac | |
| 10209A-A12 | VGB6 | C57BL/6NTac | |
| 10209A-B2 | VGB6 | C57BL/6NTac | |
| 10209A-B6 | VGB6 | C57BL/6NTac | |
| 10209A-G2 | VGB6 | C57BL/6NTac | |
