| Gene | Smtnl1 |
| Chromosome | 2 |
| Deletion Start | 84,818,905 |
| Deletion End | 84,811,376 |
| Deletion Size | 7,530 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 84,818,817 | 84,818,836 | CCAGGAAGTCAAGGCATTGC |
| TUP | 84,818,791 | 84,818,814 | CAACTGTGCCTTCGGCCACCTCCT |
| TUR | 84,818,766 | 84,818,789 | GAAGGCCCTTCTTTGTCACTTGTC |
| TDF | 84,811,572 | 84,811,592 | TTGGCTTCCTCTTCACCTTTG |
| TDP | 84,811,542 | 84,811,566 | ATGCTCCTCATTCCACCCTCCCCTC |
| TDR | 84,811,521 | 84,811,540 | GGCACAGTCGGCTAGTTTCC |
PCR Primers
| Primer | Sequence |
| SU | GTCAATATGCTCCAACTGCC |
| SD | AAAGAGACCTGGCAAACTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 340 bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 331 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10210A-C3 | VGB6 | C57BL/6NTac | |
| 10210A-C8 | VGB6 | C57BL/6NTac | |
| 10210A-C11 | VGB6 | C57BL/6NTac | |
| 10210A-G4 | VGB6 | C57BL/6NTac | |
| 10210A-G5 | VGB6 | C57BL/6NTac | |
| 10210A-H3 | VGB6 | C57BL/6NTac | |
