| Gene | C1qb |
| Chromosome | 4 |
| Deletion Start | 136,882,304 |
| Deletion End | 136,880,289 |
| Deletion Size | 2,016 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 136,881,801 | 136,881,822 | AAAAGGCAGGTGTAGGCTTGAG |
| TUP | 136,881,765 | 136,881,794 | ATCGCTTTGAAGTTCCCTCTGTAATCCAGG |
| TUR | 136,881,743 | 136,881,762 | CCACCAAGGGTGGCTTACAG |
| TDF | 136,880,575 | 136,880,593 | CACGCAACGGCAAGTTCAC |
| TDP | 136,880,546 | 136,880,573 | TGCAAGGTGCCTGGCCTCTACTACTTCA |
| TDR | 136,880,526 | 136,880,544 | CCGGGAGCTGGCATGATAG |
PCR Primers
| Primer | Sequence |
| SU | GGATTAATGACAGATGGAAG |
| SD | ACTAACACCTCTCAGGCCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 197 bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | 326 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10211A-E3 | VGB6 | C57BL/6NTac | |
| 10211A-E7 | VGB6 | C57BL/6NTac | |
| 10211A-F5 | VGB6 | C57BL/6NTac | |
| 10211A-F9 | VGB6 | C57BL/6NTac | |
| 10211A-F12 | VGB6 | C57BL/6NTac | |
