| Gene | Gstm3 |
| Chromosome | 3 |
| Deletion Start | 107,969,137 |
| Deletion End | 107,964,187 |
| Deletion Size | 4,951 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 107,968,721 | 107,968,743 | CAGACACCTGCTTTCCTCTTACC |
| TUP | 107,968,688 | 107,968,712 | CCCGTACCCAGCCCCTAATTCCTCA |
| TUR | 107,968,651 | 107,968,673 | GCTCCTTCCTCCTCCAGTTGTAG |
| TDF | 107,964,413 | 107,964,431 | GCCAGGGTTGGGAGCTTAG |
| TDP | 107,964,387 | 107,964,411 | CAAGCTCTGGACCACAGCACAGCCT |
| TDR | 107,964,357 | 107,964,377 | CCAGGTCAGGAACTGCATTTC |
PCR Primers
| Primer | Sequence |
| SU | GCTGTGGGTGAAGCCAAAGG |
| SD | ATGGGGAGAATAAAGGCTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 313 bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | 278 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10212A-B12 | VGB6 | C57BL/6NTac | |
| 10212A-E5 | VGB6 | C57BL/6NTac | |
| 10212A-E11 | VGB6 | C57BL/6NTac | |
| 10212A-H2 | VGB6 | C57BL/6NTac | |
| 10212B-A4 | VGB6 | C57BL/6NTac | |
| 10212B-B3 | VGB6 | C57BL/6NTac | |
