| Gene | Krt32 |
| Chromosome | 11 |
| Deletion Start | 100,088,085 |
| Deletion End | 100,081,159 |
| Deletion Size | 6,946 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,088,057 | 100,088,076 | TGCATGCCGACCACCTACAG |
| TUP | 100,088,022 | 100,088,053 | AGCCAGCTGCCTTTCTAAGACCTACTTGTCCA |
| TUR | 100,088,001 | 100,088,020 | GGTTGCTGGGTTGGCAAGAG |
| TDF | 100,081,384 | 100,081,404 | GGTGGAAGAGATGTGCAGTGG |
| TDP | 100,081,348 | 100,081,376 | TTGACAATTCTACCCTCAGGAGCCTCGTT |
| TDR | 100,081,326 | 100,081,346 | GGAGCCCAGCTCACAACATAC |
PCR Primers
| Primer | Sequence |
| SU | CCTCATCAGCATCTCTCAAG |
| SD | AAGAGAACCTTCTACCCAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 226 bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 322 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10218A-D9 | VGB6 | C57BL/6NTac | |
| 10218A-E4 | VGB6 | C57BL/6NTac | |
| 10218A-F3 | VGB6 | C57BL/6NTac | |
| 10218A-G1 | VGB6 | C57BL/6NTac | |
| 10218A-G2 | VGB6 | C57BL/6NTac | |
