| Gene | Inca1 |
| Chromosome | 11 |
| Deletion Start | 70,695,904 |
| Deletion End | 70,688,585 |
| Deletion Size | 7,320 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,695,846 | 70,695,867 | CCAAGTGAGTCCTCCCTAATGG |
| TUP | 70,695,821 | 70,695,844 | TCCATCCCATCCTTGTCCTTCCCA |
| TUR | 70,695,774 | 70,695,798 | TCCTGCTCTTTTGTACTCCTTTCTG |
| TDF | 70,688,897 | 70,688,917 | AGAAGCCAAGAGCCAGGTAGG |
| TDP | 70,688,863 | 70,688,894 | TTTCTTGACCACTTTTCGTCCTTAAACCAGCT |
| TDR | 70,688,838 | 70,688,858 | CAGGCACATACCCTCAAAAGC |
PCR Primers
| Primer | Sequence |
| SU | TTCGCCACCACACCCGGCTC |
| SD | AATAACAGAACAGAGAGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 304 bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | 302 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10221B-B12 | VGB6 | C57BL/6NTac | |
| 10221D-C1 | VGB6 | C57BL/6NTac | |
| 10221D-H9 | VGB6 | C57BL/6NTac | |
