| Gene | Gsdma3 |
| Chromosome | 11 |
| Deletion Start | 98,666,041 |
| Deletion End | 98,638,091 |
| Deletion Size | 27,951 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 98,629,378 | 98,629,399 | GGCCCTGGTTAGAGAGCTGAAC |
| TUP | 98,666,121 | 98,666,148 | CTGACACCCCTAGACAGCCTCATCGACT |
| TUR | 98,629,441 | 98,629,462 | GCAGAAGGGACGAAAGTGTTTG |
| TDF | 98,637,985 | 98,638,005 | GATCCAGACACTCGCCACAAC |
| TDP | 98,676,365 | 98,676,388 | TGCCCTCTATGCTGGCCTCTCCCT |
| TDR | 98,638,036 | 98,638,058 | GATTTCCTGCTTAGCAGGTGAAG |
PCR Primers
| Primer | Sequence |
| SU | AGCCGGCATCTTGTGTCAGC |
| SD | GATCTTTCAGTAACTTCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 335 bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | 230 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10224A-A4 | VGB6 | C57BL/6NTac | |
| 10224A-A9 | VGB6 | C57BL/6NTac | |
| 10224A-B12 | VGB6 | C57BL/6NTac | |
| 10224A-H4 | VGB6 | C57BL/6NTac | |
| 10224A-C8 | VGB6 | C57BL/6NTac | |
