| Gene | Cbln3 |
| Chromosome | 14 |
| Deletion Start | 55,884,184 |
| Deletion End | 55,882,961 |
| Deletion Size | 1,224 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,883,472 | 55,883,492 | AGGTGTACAACCGCCAAACTG |
| TUP | 55,883,442 | 55,883,469 | CAGGTGACCTAAGTGCCAGTCCACCTCC |
| TUR | 55,883,407 | 55,883,427 | TGTGCTGCGGTCCACTATGAC |
| TDF | 55,883,641 | 55,883,660 | CAAATGCCAACCCAGTGATG |
| TDP | 55,883,615 | 55,883,636 | TGAGCTCCCGGCACCCAGAATC |
| TDR | 55,883,566 | 55,883,586 | CTCGCCCTCATTGACCAGTAC |
PCR Primers
| Primer | Sequence |
| SU | ATCCGCAGAGACAGCAGTAG |
| SD | GAGACTGGGAGGGCGACCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 316 bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | 295 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10226A-A11 | VGB6 | C57BL/6NTac | |
| 10226A-A7 | VGB6 | C57BL/6NTac | |
| 10226A-E10 | VGB6 | C57BL/6NTac | |
