| Gene | Cbln4 |
| Chromosome | 2 |
| Deletion Start | 172,042,295 |
| Deletion End | 172,037,351 |
| Deletion Size | 4,945 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 172,042,055 | 172,042,074 | CACCAACCACGAGCCATCTG |
| TUP | 172,042,001 | 172,042,029 | CCCTCGGGAGTCTGACCTGATCAAAGTAA |
| TUR | 172,041,971 | 172,041,991 | AGACCACCCATCCCCATATGC |
| TDF | 172,038,011 | 172,038,031 | GCTGTGCAAATTCCAGAAACC |
| TDP | 172,037,983 | 172,038,009 | CTGCTCCCAGCAACTGAGAAGGCGTCT |
| TDR | 172,037,952 | 172,037,976 | ACCTAACCCTGACATGGTCAGATAG |
PCR Primers
| Primer | Sequence |
| SU | GTGCGCGCGCCGCTGCTGCC |
| SD | AGTTAACATGTAAGCAGATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 265 bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | 324 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10227B-A1 | VGB6 | C57BL/6NTac | |
| 10227B-C4 | VGB6 | C57BL/6NTac | |
| 10227B-D4 | VGB6 | C57BL/6NTac | |
| 10227B-F2 | VGB6 | C57BL/6NTac | |
| 10227B-A11 | VGB6 | C57BL/6NTac | |
| 10227D-B11 | VGB6 | C57BL/6NTac | |
| 10227D-F11 | VGB6 | C57BL/6NTac | |
