| Gene | Nags |
| Chromosome | 11 |
| Deletion Start | 102,145,570 |
| Deletion End | 102,149,071 |
| Deletion Size | 3,502 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 102,145,944 | 102,145,964 | TTCCAGACCTGCTACCATTCG |
| TUP | 102,145,966 | 102,145,988 | TGGACAAGCCCTTCGCCGTCATG |
| TUR | 102,145,991 | 102,146,009 | ACACCGGGATCGGCTTACC |
| TDF | 102,148,908 | 102,148,927 | CCACGCCCAGGTACTTCAAG |
| TDP | 102,148,929 | 102,148,957 | ACAGTGACGGTAGCTTCTCCAACAAGCAG |
| TDR | 102,148,960 | 102,148,980 | AGGCCGAACCAGAAGAAGATC |
PCR Primers
| Primer | Sequence |
| SU | GGTTCCGTACCCCCATACAC |
| SD | TCCCAGCCCCGTTTCCCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 344 bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | 311 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10229B-H2 | VGB6 | C57BL/6NTac | |
| 10229E-B5 | VGB6 | C57BL/6NTac | |
