| Gene | Hsd17b1 |
| Chromosome | 11 |
| Deletion Start | 101,078,426 |
| Deletion End | 101,080,254 |
| Deletion Size | 1,829 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 101,078,887 | 101,078,906 | AAAGAGCGGTGCACAAATGG |
| TUP | 101,078,923 | 101,078,946 | CCTCAGTCTGTAATGCTGGCCGCG |
| TUR | 101,078,948 | 101,078,967 | CTTCTAGCGGCCCAAACAAG |
| TDF | 101,079,850 | 101,079,868 | GTCTCGGGCACCGTTTCTG |
| TDP | 101,079,906 | 101,079,930 | CAGACACGCGCTGCTCTTCCTGCTT |
| TDR | 101,079,936 | 101,079,956 | GCTCGCATTGCAGTCAAGAAG |
PCR Primers
| Primer | Sequence |
| SU | CAACCCATCCCTTGATCAAC |
| SD | AACCCTTAGCCTAGCACCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 351 bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | 310 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10231B-A2 | VGB6 | C57BL/6NTac | |
| 10231B-F1 | VGB6 | C57BL/6NTac | |
| 10231B-G7 | VGB6 | C57BL/6NTac | |
| 10231B-H6 | VGB6 | C57BL/6NTac | |
| 10231B-H9 | VGB6 | C57BL/6NTac | |
| 10231B-H11 | VGB6 | C57BL/6NTac | |
