| Gene | Plekhh3 |
| Chromosome | 11 |
| Deletion Start | 103,983,769 |
| Deletion End | 103,969,101 |
| Deletion Size | 8,024 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 101,170,683 | 101,170,706 | AGAACCGAGTTCAATCTTTCTCAC |
| TUP | 101,170,663 | 101,170,682 | TACAACCGGCCGTCACGCCT |
| TUR | 101,170,632 | 101,170,652 | ACTGCGTTTGGCCCAAGTATG |
| TDF | 101,163,656 | 101,163,674 | GAAGCTCTGCCTGGGTCTG |
| TDP | 101,163,631 | 101,163,653 | AGCAAAGGCCATGTCGCTCTCCA |
| TDR | 101,163,600 | 101,163,622 | ACACTGTGGATGGGTTCTGTCTC |
PCR Primers
| Primer | Sequence |
| SU | TATCTCCCCCCTCCTCTGCC |
| SD | AACAAACTAGAAAACTGTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 2812810 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | 2806479 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10234C-A9 | VGB6 | C57BL/6NTac | |
| 10234C-C8 | VGB6 | C57BL/6NTac | |
| 10234C-G10 | VGB6 | C57BL/6NTac | |
