| Gene | Cited4 |
| Chromosome | 4 |
| Deletion Start | 120,666,786 |
| Deletion End | 120,667,351 |
| Deletion Size | 566 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,666,971 | 120,666,989 | CTGGCATACGGCTCCTTCG |
| TUP | 120,667,001 | 120,667,024 | TCTTTCCAGCCCTTCCCCGTCTCG |
| TUR | 120,667,040 | 120,667,058 | ACTGCAGGTGCGTGCTACC |
| TDF | 120,667,197 | 120,667,216 | CCGAGCTCATCGACGAAGAG |
| TDP | 120,667,272 | 120,667,296 | AGCTCTTCCTCGGCCAGAGCGAGTT |
| TDR | 120,667,298 | 120,667,318 | CCCCAGGTCTGAGAAGCAATC |
PCR Primers
| Primer | Sequence |
| SU | TTGCGGCCTGCAACTACTGC |
| SD | ATCTGGCAGGAGGCAGAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 310 bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 122 bp | + | + | - |
| NeoFwd | SD | 229 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10235B-G11 | VGB6 | C57BL/6NTac | |
| 10235C-A3 | VGB6 | C57BL/6NTac | |
| 10235C-B5 | VGB6 | C57BL/6NTac | |
| 10235C-C2 | VGB6 | C57BL/6NTac | |
| 10235C-E1 | VGB6 | C57BL/6NTac | |
| 10235C-G4 | VGB6 | C57BL/6NTac | |
