| Gene | Ifit2 |
| Chromosome | 19 |
| Deletion Start | 34,570,349 |
| Deletion End | 34,574,474 |
| Deletion Size | 4,126 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,570,386 | 34,570,409 | TTCTGGTGATTGTGGGAATTGTAG |
| TUP | 34,570,416 | 34,570,445 | TTGATGACAGATGCAAATCCTAAACAGCCA |
| TUR | 34,570,452 | 34,570,476 | AAACACAGAGGACTGGTCTCTCTTG |
| TDF | 34,574,304 | 34,574,322 | GCCCTAAGGCGGCTTCATG |
| TDP | 34,574,333 | 34,574,360 | TGCCAAGATATGCAAGGCCTCAGAATCA |
| TDR | 34,574,372 | 34,574,392 | GCTTGCTGACCTCCTCCATTC |
PCR Primers
| Primer | Sequence |
| SU | CTGACCCTGAAAGGCTTGGCTC |
| SD | AGATCCTGTATCTATCACCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 358 bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | 301 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10236A-A5 | VGB6 | C57BL/6NTac | |
| 10236A-A6 | VGB6 | C57BL/6NTac | |
| 10236A-D9 | VGB6 | C57BL/6NTac | |
| 10236B-B4 | VGB6 | C57BL/6NTac | |
