| Gene | 1700034O15Rik |
| Chromosome | 6 |
| Deletion Start | 41,684,521 |
| Deletion End | 41,685,390 |
| Deletion Size | 870 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 41,685,262 | 41,685,283 | GGTGAGCAGTGGCAGGAGATAG |
| TUP | 41,685,287 | 41,685,313 | TGGTCCAGCAAGAAGACACGATCTCCC |
| TUR | 41,685,316 | 41,685,337 | GGTGGTCCTGGAGAGTTATTGC |
| TDF | 41,684,545 | 41,684,568 | AGAGCAACATTCCTGACAGCTTTG |
| TDP | 41,684,570 | 41,684,596 | CCTTCACTTGGAGCAGCAGCAGTAGCA |
| TDR | 41,684,601 | 41,684,627 | AAGCTTGCACTCACTATCAGAGTCTTC |
PCR Primers
| Primer | Sequence |
| SU | ATGACCATCTCAGATGCCTC |
| SD | TGTCTTTGGCCCCGGGACATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 372 bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | 337 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10238B-D2 | VGB6 | C57BL/6NTac | |
| 10238B-D3 | VGB6 | C57BL/6NTac | |
| 10238B-F2 | VGB6 | C57BL/6NTac | |
| 10238B-F9 | VGB6 | C57BL/6NTac | |
| 10238B-G10 | VGB6 | C57BL/6NTac | |
| 10238B-H1 | VGB6 | C57BL/6NTac | |
