| Gene | Mup4 |
| Chromosome | 4 |
| Deletion Start | 59,960,596 |
| Deletion End | 59,957,865 |
| Deletion Size | 2,732 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 62,014,269 | 62,014,293 | TTGTGTATGCTCAGACCCTAATGC |
| TUP | 59,959,979 | 59,960,006 | TGCATGTTTCCACTTGAACTCCAGCCT |
| TUR | 59,959,958 | 59,959,977 | GGGAGAAGGAGCAGGGATACC |
| TDF | 60,005,770 | 60,005,793 | TGGATGCCAAATCCAGAATAGATC |
| TDP | 59,958,251 | 59,958,276 | CACCCATGCCTGTCAATCATGTTGCC |
| TDR | 59,958,221 | 59,958,241 | GGGCTTCCCCATAAGGATGTG |
PCR Primers
| Primer | Sequence |
| SU | TTTGAGAAATGTAAGACTAC |
| SD | TTGTTTTCTTGCCTAAGTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 1923147 bp | - | + | + |
| TUF | TUR | 2054336 bp | + | + | - |
| TDF | TDR | 47573 bp | + | + | - |
| NeoFwd | SD | 1916696 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10241B-E2 | VGB6 | C57BL/6NTac | |
| 10241B-G7 | VGB6 | C57BL/6NTac | |
