| Gene | 1700003F12Rik |
| Chromosome | 2 |
| Deletion Start | 154,548,926 |
| Deletion End | 154,549,964 |
| Deletion Size | 1,039 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 154,549,018 | 154,549,037 | AAGCCCGTCACAAGCAGTTC |
| TUP | 154,549,039 | 154,549,064 | TCAGCCATCTCAAGAGGAAGAAGCCA |
| TUR | 154,549,072 | 154,549,093 | GTCCCTTCTAGCCTCTCCATAC |
| TDF | 154,549,535 | 154,549,554 | CCCAGACCAAGATCGTGCTG |
| TDP | 154,549,555 | 154,549,578 | CTTTTCCCGCTGGACAAGCGGCAG |
| TDR | 154,549,604 | 154,549,621 | GCACCGGCCTCACAAATG |
PCR Primers
| Primer | Sequence |
| SU | CTAGCCCTGTTTTGCAGCTG |
| SD | TCAAGTCCACCTTCAGTCTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 356 bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | 291 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10243A-C10 | VGB6 | C57BL/6NTac | |
| 10243A-E8 | VGB6 | C57BL/6NTac | |
