10249   Endou     Status: ES cell clone microinjected

GeneEndou
Chromosome15
Deletion Start97,721,138
Deletion End97,712,034
Deletion Size9,105
Genome Build38
MGI IDMGI:97746
NCBI Gene ID19011
BAC ID429H5
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 15

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF97,720,70397,720,725GATCCAGAGGACAAGATCCCAAC
TUP97,720,68097,720,702CAGCCGTAAGCCTGAAGCCCAGA
TUR97,720,58997,720,609TGGACAGGTCCAGATTGATTC
TDF97,712,21697,712,236CCCATGTGGAACAGACGTAAG
TDP97,712,18197,712,210TTGTGGGCTCCACTAATTCAGTTCATGATC
TDR97,712,14497,712,162GATAGCCACCCAGGCTCAG

PCR Primers

PrimerSequence
SUCCATTAGGGAGTCAAACTGTC
SDGTGGGCATTTCTAGATTCGAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev353 bp-++
TUFTUR137 bp++-
TDFTDR93 bp++-
NeoFwdSD233 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
10249B-B12VGB6C57BL/6NTac
10249B-E2VGB6C57BL/6NTac
10249B-G3VGB6C57BL/6NTac
10249B-H3VGB6C57BL/6NTac
10249C-G12VGB6C57BL/6NTac
10249C-E8VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
Larger Image

VelociGene Genotyping Strategy

Larger Image