| Gene | Oog2 |
| Chromosome | 4 |
| Deletion Start | 144,193,996 |
| Deletion End | 144,196,594 |
| Deletion Size | 2,620 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 144,194,009 | 144,194,028 | ATTCGCCACCCACTCTGATG |
| TUP | 144,194,034 | 144,194,063 | AGCAAGACAGTGTCTACTGAGGGATGAGTA |
| TUR | 144,194,070 | 144,194,092 | GCAGGTCCTTGAGAGCAGAAATG |
| TDF | 144,195,803 | 144,195,824 | TGCCAGGTCCTGAATCATGTTG |
| TDP | 144,195,828 | 144,195,856 | TGGACAGGCCTCAGACTAGAATGATCTGA |
| TDR | 144,195,860 | 144,195,881 | GCCTTCTGTTGTCTGTTCACAC |
PCR Primers
| Primer | Sequence |
| SU | TGTGGAGTTCAGGCCAGTG |
| SD | GTATGCAGTCAACAGATGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 387 bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 349 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10250A-H12 | VGB6 | C57BL/6NTac | |
