| Gene | Bhlha9 |
| Chromosome | 11 |
| Deletion Start | 76,672,723 |
| Deletion End | 76,673,267 |
| Deletion Size | 545 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,672,816 | 76,672,835 | CATGACCTCGGAGGCAAGAG |
| TUP | 76,672,838 | 76,672,860 | TCTCCAAGATCGCTACGCTGCGC |
| TUR | 76,672,885 | 76,672,904 | GCACGCAGGACCAAGGATAG |
| TDF | 76,673,030 | 76,673,047 | TAACGCGCAGGGACATCG |
| TDP | 76,673,053 | 76,673,074 | CCCTTAGTGCCGCCTACACCGC |
| TDR | 76,673,121 | 76,673,141 | AAATTGGGCACTTCGGCCATC |
PCR Primers
| Primer | Sequence |
| SU | GACCAGCAGCCTTTTGGAC |
| SD | GAAAAGGAGGGCTCAGAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 361 bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 112 bp | + | + | - |
| NeoFwd | SD | 334 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10252A-A11 | VGB6 | C57BL/6NTac | |
| 10252A-A12 | VGB6 | C57BL/6NTac | |
| 10252B-D12 | VGB6 | C57BL/6NTac | |
