| Gene | Slc46a2 |
| Chromosome | 4 |
| Deletion Start | 59,914,918 |
| Deletion End | 59,906,362 |
| Deletion Size | 8,557 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 59,914,309 | 59,914,331 | CAAGCAAATCGTTGGGCACTCTG |
| TUP | 59,914,286 | 59,914,308 | CGCAAGGCCTCCTGTTAACTGCC |
| TUR | 59,914,256 | 59,914,274 | AAGGGCAAACGCCGCACAG |
| TDF | 59,906,946 | 59,906,969 | GGAGAATGATTTGGGACCATGAAG |
| TDP | 59,906,916 | 59,906,945 | ACGAGTTGGTAAATGCTTGCTGTGTAAGCG |
| TDR | 59,906,889 | 59,906,911 | GTGCTGAAGACCTGACTCGTATC |
PCR Primers
| Primer | Sequence |
| SU | GACCCAGGTGGAGGTGGAGG |
| SD | TGATTTTCCCTCCCACCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 349 bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | 176 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10257A-C3 | VGB6 | C57BL/6NTac | |
| 10257A-F2 | VGB6 | C57BL/6NTac | |
