| Gene | Rimkla |
| Chromosome | 4 |
| Deletion Start | 119,492,456 |
| Deletion End | 119,468,049 |
| Deletion Size | 24,408 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 119,491,967 | 119,491,990 | GGTATGTATGCTCCAGTGATGAAC |
| TUP | 119,491,932 | 119,491,961 | AGGTAGAACACTATTCTCAAACTGGGAGCC |
| TUR | 119,491,889 | 119,491,911 | TCCAGAGACGATCTCAATCTTTC |
| TDF | 119,468,590 | 119,468,611 | GTCACCTGTCCCATCTTTCTTG |
| TDP | 119,468,561 | 119,468,589 | AAGGTCATTAGCCTCATCAGTGAGTGGCA |
| TDR | 119,468,511 | 119,468,528 | TGACACCCACACCACCTG |
PCR Primers
| Primer | Sequence |
| SU | CGTCGCGACGTGCTGATTG |
| SD | GAAGTTGTTCTCCATGAGTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 318 bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | 323 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10262B-A12 | VGB6 | C57BL/6NTac | |
| 10262D-A10 | VGB6 | C57BL/6NTac | |
