| Gene | Clvs1 |
| Chromosome | 4 |
| Deletion Start | 9,281,542 |
| Deletion End | 9,282,006 |
| Deletion Size | 465 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 9,281,565 | 9,281,588 | CAGTTTCTGTTCTTCCCTCACCTC |
| TUP | 9,281,593 | 9,281,621 | CTTAAGCACTTGGGAAGGAGATTTGGCCA |
| TUR | 9,281,630 | 9,281,648 | TGAGCCCAGCCTGCAAATG |
| TDF | 9,281,915 | 9,281,935 | AGGGCTTTGATCGACGGATTC |
| TDP | 9,281,936 | 9,281,960 | CCCGGAGTGCTGGAAAATCGTGACC |
| TDR | 9,281,978 | 9,281,996 | TGGCAGCAAACAGCAAGAG |
PCR Primers
| Primer | Sequence |
| SU | ATGCTCATCAGACCCACACTTC |
| SD | CCTTTTGGAACATCAACTTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 406 bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | 344 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10263A-F9 | VGB6 | C57BL/6NTac | |
| 10263B-D1 | VGB6 | C57BL/6NTac | |
