| Gene | Alg2 |
| Chromosome | 4 |
| Deletion Start | 47,474,283 |
| Deletion End | 47,471,539 |
| Deletion Size | 2,745 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 47,474,143 | 47,474,165 | AGGAGTACGGCTGTGATGTGAAG |
| TUP | 47,474,119 | 47,474,142 | ATATGGACCGCGCACTACGACCCG |
| TUR | 47,474,093 | 47,474,110 | TCGCGGGTCTCGATGAAG |
| TDF | 47,472,073 | 47,472,090 | TCTGCCCTTGGCACTGAG |
| TDP | 47,472,048 | 47,472,071 | TCCTTGGTGCAGCTTCGGAATCGG |
| TDR | 47,471,999 | 47,472,017 | AACCACCGGCCATGAAGAG |
PCR Primers
| Primer | Sequence |
| SU | GTCAGGCATTTTCTTAGCGC |
| SD | GGAATGATTACAGCATTAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 280 bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | 303 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10264A-B5 | VGB6 | C57BL/6NTac | |
| 10264D-C11 | VGB6 | C57BL/6NTac | |
| 10264D-H10 | VGB6 | C57BL/6NTac | |
