| Gene | Gstm6 |
| Chromosome | 3 |
| Deletion Start | 107,943,630 |
| Deletion End | 107,939,231 |
| Deletion Size | 4,400 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 107,942,876 | 107,942,895 | GCAGCTTTGGTTGGAAAGAC |
| TUP | 107,942,847 | 107,942,873 | ACTGGGTCAGACTGCATATTCCTTGCA |
| TUR | 107,942,819 | 107,942,840 | CACAAGAAGTCAAAGGCAGGAC |
| TDF | 107,940,024 | 107,940,042 | GCTCTCATCACTGCGGTTG |
| TDP | 107,939,998 | 107,940,023 | TGATCCACATGGAGTGGCCTTGTGAG |
| TDR | 107,939,950 | 107,939,968 | AACAAGGGCCTCTGCACTC |
PCR Primers
| Primer | Sequence |
| SU | CAATCAATCACTGCCTTCGG |
| SD | GCAGAAAGAAACAAGGGAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 370 bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | 346 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10267A-B12 | VGB6 | C57BL/6NTac | |
| 10267A-C3 | VGB6 | C57BL/6NTac | |
| 10267A-E8 | VGB6 | C57BL/6NTac | |
| 10267A-F3 | VGB6 | C57BL/6NTac | |
| 10267A-G10 | VGB6 | C57BL/6NTac | |
| 10267A-H1 | VGB6 | C57BL/6NTac | |
