| Gene | Prpf38b |
| Chromosome | 3 |
| Deletion Start | 108,911,439 |
| Deletion End | 108,903,919 |
| Deletion Size | 7,542 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 108,910,543 | 108,910,563 | GGAAGTCAGGAGTCGAAGGTG |
| TUP | 108,910,520 | 108,910,541 | CAGCCACCTGTGCGTTGGGCAT |
| TUR | 108,910,476 | 108,910,495 | GACCCTGCCGCAGAAAGAAG |
| TDF | 108,904,581 | 108,904,602 | AGGCGAAGATCCCGAAGCATTG |
| TDP | 108,904,550 | 108,904,573 | CTTAGATCGCAGGCGAAGCCGCAG |
| TDR | 108,904,481 | 108,904,503 | TCCCGATCCCTGTCTCTTCTATC |
PCR Primers
| Primer | Sequence |
| SU | AGATCGAGCTCCTCTGCTG |
| SD | ACATTCTGTGCATCATGTGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 373 bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 122 bp | + | + | - |
| NeoFwd | SD | 305 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10281A-A9 | VGB6 | C57BL/6NTac | |
| 10281A-B1 | VGB6 | C57BL/6NTac | |
| 10281A-B3 | VGB6 | C57BL/6NTac | |
| 10281A-C12 | VGB6 | C57BL/6NTac | |
| 10281A-H2 | VGB6 | C57BL/6NTac | |
