| Gene | Rhbdl1 |
| Chromosome | 17 |
| Deletion Start | 25,837,023 |
| Deletion End | 25,834,753 |
| Deletion Size | 2,271 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,836,723 | 25,836,746 | AGAGCGAATATGAGGTGGTAGAAC |
| TUP | 25,836,701 | 25,836,722 | AGCCAAACCAGGCACCTTGCCC |
| TUR | 25,836,642 | 25,836,662 | GGACCTTTGTGCCAGTCAGTG |
| TDF | 25,835,560 | 25,835,580 | TGGAGCAGCTAGGGTTCAATG |
| TDP | 25,835,536 | 25,835,559 | CCCTCCTGCAGCTGATGATCGGTG |
| TDR | 25,835,507 | 25,835,527 | AAGCACGCCATGTACCATCTC |
PCR Primers
| Primer | Sequence |
| SU | GGCTGCCAGCTATGGCCTAC |
| SD | ACCCCTCCCCCTGGAAAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 433 bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | 263 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10283A-B12 | VGB6 | C57BL/6NTac | |
| 10283A-C6 | VGB6 | C57BL/6NTac | |
| 10283A-F3 | VGB6 | C57BL/6NTac | |
| 10283A-G2 | VGB6 | C57BL/6NTac | |
| 10283A-G11 | VGB6 | C57BL/6NTac | |
| 10283A-H11 | VGB6 | C57BL/6NTac | |
