| Gene | Rtp4 |
| Chromosome | 16 |
| Deletion Start | 23,609,973 |
| Deletion End | 23,613,490 |
| Deletion Size | 3,518 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 23,610,068 | 23,610,090 | GGAGCCTGCATTTGGATAAGAAC |
| TUP | 23,610,093 | 23,610,115 | TGTACCAGATGGTGCAGCCCTGG |
| TUR | 23,610,135 | 23,610,152 | CACCTGCCAAGCACTGTC |
| TDF | 23,612,844 | 23,612,864 | GCCAAGGACACAGAGGATTTC |
| TDP | 23,612,870 | 23,612,899 | TCTGTCTATAGGTTCCAGTGTTCCAGATGC |
| TDR | 23,612,904 | 23,612,923 | CTGAGCAGAGGTCCAACTTC |
PCR Primers
| Primer | Sequence |
| SU | AACCAGAACTCCGCAGTTTC |
| SD | ATACGTGAGCAAGCTAGTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 342 bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | 283 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10286A-B10 | VGB6 | C57BL/6NTac | |
| 10286A-E3 | VGB6 | C57BL/6NTac | |
