| Gene | Gatsl3 |
| Chromosome | 11 |
| Deletion Start | 4,218,298 |
| Deletion End | 4,222,130 |
| Deletion Size | 3,833 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,218,954 | 4,218,974 | GTTCTTCAGCCTGACTGAGAC |
| TUP | 4,218,976 | 4,219,004 | CCGGAAGATTACACTCTCATGGTGGATGA |
| TUR | 4,219,026 | 4,219,047 | TTAGAGGCAAACAAGGAACCAG |
| TDF | 4,221,608 | 4,221,627 | TGGGATTGTGGCCCAGATTG |
| TDP | 4,221,634 | 4,221,656 | CCCTGGCGGCTGTTGACATCTCT |
| TDR | 4,221,677 | 4,221,696 | CCAGCGCATGGTCAAAGTTG |
PCR Primers
| Primer | Sequence |
| SU | GTGAGGGCTAGAAGAGTGGC |
| SD | AGATTTAATTCTTCCTTCGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 349 bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | 303 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10288A-B1 | VGB6 | C57BL/6NTac | |
| 10288B-B3 | VGB6 | C57BL/6NTac | |
| 10288B-D12 | VGB6 | C57BL/6NTac | |
| 10288B-G4 | VGB6 | C57BL/6NTac | |
