| Gene | Spata2 |
| Chromosome | 2 |
| Deletion Start | 167,485,472 |
| Deletion End | 167,483,331 |
| Deletion Size | 2,142 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 167,484,945 | 167,484,966 | TCCTGGCATGTGCATTTGTTTG |
| TUP | 167,484,910 | 167,484,937 | TCGTGCTAGGGATTAAACCTGTAGTGCA |
| TUR | 167,484,879 | 167,484,899 | CAAGGCTGGGATGTAGCTCAG |
| TDF | 167,483,547 | 167,483,567 | AGCCCAAGCCCTCTACCACAG |
| TDP | 167,483,524 | 167,483,546 | CCACCTCCCGCTGTGGCTTCTGT |
| TDR | 167,483,464 | 167,483,481 | GCAGGCGTCACAGGAAAC |
PCR Primers
| Primer | Sequence |
| SU | GCCAGCTTACTCTAGGCCAG |
| SD | GCAGCGAAGTGACTGAATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 339 bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | 309 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10289A-E1 | VGB6 | C57BL/6NTac | |
| 10289A-F6 | VGB6 | C57BL/6NTac | |
| 10289B-A12 | VGB6 | C57BL/6NTac | |
