10294   Fdxr     Status: ES cell colonies screened / QC positives

GeneFdxr
Chromosome11
Deletion Start115,276,884
Deletion End115,268,305
Deletion Size8,580
Genome Build38
MGI IDMGI:104724
NCBI Gene ID14149
BAC ID104E11
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF115,276,672115,276,691TTGCGGGAGAAATGGGAGTG
TUP115,276,631115,276,660AGGATCTGTGCAAAGAGATGTAAGGCGTCC
TUR115,276,606115,276,628AGCCTTTACTGAGCTGTCTTACC
TDF115,268,455115,268,477GCCCATCTTTGTATTCGCCTCAG
TDP115,268,427115,268,451TCCGGCCAGTGTCTTTCTCAGACTG
TDR115,268,405115,268,425TTCCTCAGCATCCAGCTTCTC

PCR Primers

PrimerSequence
SUCTTTTGAGGATTGGACGTTC
SDGAGGTCAGCTTGCAGAAGGC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev368 bp-++
TUFTUR86 bp++-
TDFTDR73 bp++-
NeoFwdSD235 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
10294A-B10VGB6C57BL/6NTac
10294E-C2VGB6C57BL/6NTac
10294C-E3VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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