| Gene | Tmem88b |
| Chromosome | 4 |
| Deletion Start | 155,785,599 |
| Deletion End | 155,784,247 |
| Deletion Size | 1,353 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 155,785,566 | 155,785,590 | GAAAGGGAGACAGAAGAGGATGAAG |
| TUP | 155,785,541 | 155,785,564 | CGTTGCCTCAGACACAGCACCCAT |
| TUR | 155,785,492 | 155,785,510 | AGGAGCCAGGACTGAGATG |
| TDF | 155,784,653 | 155,784,671 | TCTGTCCAGGCTCCACCTC |
| TDP | 155,784,632 | 155,784,652 | CAGGCAGGCCTCCCAGCCTTA |
| TDR | 155,784,605 | 155,784,624 | TCAGGGCAGAGTCCAGAAAG |
PCR Primers
| Primer | Sequence |
| SU | ACCTGAGCCGAGCACATAG |
| SD | AGTCGGCTGCTGACCACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 402 bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | 295 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10297C-A6 | VGB6 | C57BL/6NTac | |
| 10297C-B9 | VGB6 | C57BL/6NTac | |
| 10297C-D2 | VGB6 | C57BL/6NTac | |
| 10297C-F7 | VGB6 | C57BL/6NTac | |
