| Gene | Rarres2 |
| Chromosome | 6 |
| Deletion Start | 48,572,264 |
| Deletion End | 48,569,934 |
| Deletion Size | 2,331 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 48,572,135 | 48,572,154 | GTTCCACAAACACCCACCTG |
| TUP | 48,572,110 | 48,572,134 | TGCAGTTGGCCTTCCAAGAGATCGG |
| TUR | 48,572,072 | 48,572,096 | CAATCACACCACTAACCACTTCTTC |
| TDF | 48,570,390 | 48,570,409 | CACTACATCGCCCACTCCAG |
| TDP | 48,570,366 | 48,570,389 | AGCATGCCAGAGGCTAGTCTCCAC |
| TDR | 48,570,322 | 48,570,340 | CAGGCATTTCCGCCTTCTC |
PCR Primers
| Primer | Sequence |
| SU | GGCTATAGTGGACATAACCAG |
| SD | GGTGCTGCCACAGCTGTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 368 bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | 266 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10298A-C12 | VGB6 | C57BL/6NTac | |
| 10298A-D5 | VGB6 | C57BL/6NTac | |
| 10298A-E5 | VGB6 | C57BL/6NTac | |
| 10298A-E9 | VGB6 | C57BL/6NTac | |
| 10298A-E10 | VGB6 | C57BL/6NTac | |
| 10298A-F12 | VGB6 | C57BL/6NTac | |
