| Gene | Pramef12 |
| Chromosome | 4 |
| Deletion Start | 144,395,969 |
| Deletion End | 144,392,570 |
| Deletion Size | 3,400 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 144,395,123 | 144,395,143 | GTTCTGGATTTGCGGGCTTTG |
| TUP | 144,395,097 | 144,395,122 | CACAAGGACTTCTGGACCGTATGGGC |
| TUR | 144,395,071 | 144,395,092 | GAGCGCAAGATGTTGTCGTATC |
| TDF | 144,393,012 | 144,393,031 | GCATCCGACAGCTCAAACAC |
| TDP | 144,392,984 | 144,393,010 | TGAACCTGAGTGGTGTCATTCTGACCG |
| TDR | 144,392,957 | 144,392,975 | GGACTCGAAGAGGCTCAGG |
PCR Primers
| Primer | Sequence |
| SU | ACTTCCTGCTTCCTTGGAGC |
| SD | CCACCCATGAAGTAAACACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 354 bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | 369 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10301E-A6 | VGB6 | C57BL/6NTac | |
| 10301E-C4 | VGB6 | C57BL/6NTac | |
| 10301E-H4 | VGB6 | C57BL/6NTac | |
