| Gene | 1810030O07Rik |
| Chromosome | X |
| Deletion Start | 12,673,538 |
| Deletion End | 12,657,843 |
| Deletion Size | 15,696 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 12,673,157 | 12,673,178 | CACCTCTGCCCTCTGTAGTTTC |
| TUP | 12,673,132 | 12,673,156 | AGCCTCAGTGTCAGGTATGCGCAGA |
| TUR | 12,673,081 | 12,673,101 | TGCACGTCTCCATTTCTGTTG |
| TDF | 12,658,262 | 12,658,281 | TGCCATCCACTCAACCTCTG |
| TDP | 12,658,237 | 12,658,261 | TGTAGATGCTTGCCAGTAGTCCGCA |
| TDR | 12,658,191 | 12,658,210 | GCCCTAAACCACCTGTTCTG |
PCR Primers
| Primer | Sequence |
| SU | GCTGCCACTTCCTGGAGCGG |
| SD | ACCATTCCCAACCTTCCTTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 288 bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | 216 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10302A-A10 | VGB6 | C57BL/6NTac | |
| 10302A-A11 | VGB6 | C57BL/6NTac | |
| 10302A-B2 | VGB6 | C57BL/6NTac | |
| 10302A-E6 | VGB6 | C57BL/6NTac | |
| 10302B-B5 | VGB6 | C57BL/6NTac | |
| 10302B-E5 | VGB6 | C57BL/6NTac | |
| 10302B-B6 | VGB6 | C57BL/6NTac | |
