| Gene | Tspyl3 |
| Chromosome | 2 |
| Deletion Start | 153,225,277 |
| Deletion End | 153,224,292 |
| Deletion Size | 986 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 153,225,091 | 153,225,110 | CGAGAAGGGCTCGGGTAATG |
| TUP | 153,225,054 | 153,225,082 | ACCAAAGGAAAGACTGAGGAAGTGACGAC |
| TUR | 153,225,033 | 153,225,052 | GCGGCTACAGAGTCTTCCTC |
| TDF | 153,224,465 | 153,224,485 | TTCTAGGAGGCTATGCTGTGA |
| TDP | 153,224,499 | 153,224,521 | AAGCTTCGCACGGCATCCCGGTT |
| TDR | 153,224,590 | 153,224,605 | TGCAGAGCCTCAGGCG |
PCR Primers
| Primer | Sequence |
| SU | TCATCTTCCCTGTCTCCTGC |
| SD | TGACAGTATACATCCATAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 332 bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 141 bp | + | + | - |
| NeoFwd | SD | 313 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10303A-D6 | VGB6 | C57BL/6NTac | |
| 10303A-G9 | VGB6 | C57BL/6NTac | |
