| Gene | Ly6g5b |
| Chromosome | 17 |
| Deletion Start | 35,115,397 |
| Deletion End | 35,114,395 |
| Deletion Size | 1,003 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,115,205 | 35,115,223 | TGCCACCTGTGCCTCTTAG |
| TUP | 35,115,177 | 35,115,204 | AAGACCCTTCGCTAGGCTGCATTTCTGG |
| TUR | 35,115,142 | 35,115,161 | ACGGCGATGGAAGGCTGATG |
| TDF | 35,114,691 | 35,114,712 | TGATTGCTGCCAGTACGATTAC |
| TDP | 35,114,665 | 35,114,688 | CAACTCCTGGTCCAGTGCTCAGCA |
| TDR | 35,114,622 | 35,114,641 | CATGCCCAGATGGCTTCCTG |
PCR Primers
| Primer | Sequence |
| SU | CAGGCCTTCTTCCTGCC |
| SD | GGCATATGACCAGACTGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 308 bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | 361 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10304A-B12 | VGB6 | C57BL/6NTac | |
| 10304A-E4 | VGB6 | C57BL/6NTac | |
| 10304A-E9 | VGB6 | C57BL/6NTac | |
| 10304A-F4 | VGB6 | C57BL/6NTac | |
| 10304A-G1 | VGB6 | C57BL/6NTac | |
| 10304A-G6 | VGB6 | C57BL/6NTac | |
| 10304A-H6 | VGB6 | C57BL/6NTac | |
