| Gene | Ebna1bp2 |
| Chromosome | 4 |
| Deletion Start | 118,620,916 |
| Deletion End | 118,625,870 |
| Deletion Size | 4,955 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 118,621,406 | 118,621,426 | TGGGTTGAAAGGCTCGATGTG |
| TUP | 118,621,427 | 118,621,450 | ACCCTGGGTCCAGTGCCAGAAGTC |
| TUR | 118,621,464 | 118,621,484 | GGTCCTGGTTCTGAGGTGTTG |
| TDF | 118,625,104 | 118,625,124 | GCAGGAGGCACCAATTACAAG |
| TDP | 118,625,125 | 118,625,150 | TTGGCATTTCTAAGGAACTGGAGCCA |
| TDR | 118,625,165 | 118,625,186 | CTGTCTAGCCAAACCATGTCAC |
PCR Primers
| Primer | Sequence |
| SU | TACGTAGGGATCAGTAGGTG |
| SD | GAGGCATGGCCAACAAACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 342 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | 459 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10307A-D3 | VGB6 | C57BL/6NTac | |
| 10307A-F2 | VGB6 | C57BL/6NTac | |
