| Gene | C630004H02Rik |
| Chromosome | 11 |
| Deletion Start | 115,367,606 |
| Deletion End | 115,348,448 |
| Deletion Size | 19,159 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 115,367,197 | 115,367,216 | CTCGCTTAGCCAGCCTCTTC |
| TUP | 115,367,173 | 115,367,196 | CCGCTCCCACCTACCCATCCATCG |
| TUR | 115,367,094 | 115,367,114 | CCCGGCTTTGTTTGCATTGAG |
| TDF | 115,348,536 | 115,348,557 | TGATGACAATGCCCTTCCTCTG |
| TDP | 115,348,503 | 115,348,529 | TGTGGACCCACCTATCTGGTATGACAC |
| TDR | 115,348,470 | 115,348,490 | CACACCCGCTGGATCTCAAAC |
PCR Primers
| Primer | Sequence |
| SU | TGGGTTCCACGGAGAACTCC |
| SD | TAGAACAGCCTGGAGGGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 358 bp | - | + | + |
| TUF | TUR | 123 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | 287 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10308A-A6 | VGB6 | C57BL/6NTac | |
| 10308A-D1 | VGB6 | C57BL/6NTac | |
| 10308B-G10 | VGB6 | C57BL/6NTac | |
